Abstract
Aim: UVA radiation drives skin photoaging in the dermis, plausibly via persistent changes to DNA methylation in dermal fibroblasts. Methods: Genome-wide DNA methylation changes after five repeated daily UVA doses were determined at 48 h (transitionary) and 1 week (recovery) post final irradiation. Results: Differential methylation was found at the transitionary time point in active chromatin states near genes that are highly expressed in fibroblasts and are involved in cellular defensive mechanisms; the majority of these methylation differences were restored to control levels after 7 day recovery. At the recovery time point, new differential methylation occurred at repressed regions near developmental genes, normally weakly expressed in fibroblasts. Conclusion: UVA irradiation induces transitionary and recovery-associated DNA methylation responses in fibroblasts with contrasting functional characteristics.
Overview publication
Title | Repeat UVA exposure of human skin fibroblasts induces both a transitionary and recovery DNA methylation response. |
Date | April 1st, 2020 |
Issue name | Epigenomics |
Issue number | v12.7:563-573 |
DOI | 10.2217/epi-2019-0251 |
PubMed | 32516006 |
Authors | |
Keywords | UVA irradiation, dermis, fibroblasts, genome-wide DNA methylation, photoaging, skin aging |
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